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Fig. 2. The effect of PNU282987 <t>on</t> <t>IL-22</t> production. (A) The expression level of α7nAChR in liver tissue was detected by immunohistochemistry (scale, 100 μm, 50um). (B) The integrated optical density (IOD) of α7nAChR. (C) Western blot analysis was performed to quantify the levels of α7nAChR and IL-22RA1 in liver tissue. (D and E) Densitometric analysis of protein bands in liver tissue. (F) The level of IL-22 in serum was detected by ELISA. (G) Western blot analysis was performed to quantify the levels of α7nAChR and IL-22RA1 in L-02 cells. (H and I) Densitometric analysis of protein bands in L-02 cells. (J) The level of IL-22 in cell supernatant was detected by ELISA. (K and L) qRT-PCR was used to measure the levels of α7nAChR and IL-22 in L-02 cells. (ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001).
Recombinant Human Il 22 Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+il-22+protein/IL-22%2C+Human/pm39705885-46-0-7
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Fig. 2. The effect of PNU282987 <t>on</t> <t>IL-22</t> production. (A) The expression level of α7nAChR in liver tissue was detected by immunohistochemistry (scale, 100 μm, 50um). (B) The integrated optical density (IOD) of α7nAChR. (C) Western blot analysis was performed to quantify the levels of α7nAChR and IL-22RA1 in liver tissue. (D and E) Densitometric analysis of protein bands in liver tissue. (F) The level of IL-22 in serum was detected by ELISA. (G) Western blot analysis was performed to quantify the levels of α7nAChR and IL-22RA1 in L-02 cells. (H and I) Densitometric analysis of protein bands in L-02 cells. (J) The level of IL-22 in cell supernatant was detected by ELISA. (K and L) qRT-PCR was used to measure the levels of α7nAChR and IL-22 in L-02 cells. (ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001).
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Fig. 2. The effect of PNU282987 <t>on</t> <t>IL-22</t> production. (A) The expression level of α7nAChR in liver tissue was detected by immunohistochemistry (scale, 100 μm, 50um). (B) The integrated optical density (IOD) of α7nAChR. (C) Western blot analysis was performed to quantify the levels of α7nAChR and IL-22RA1 in liver tissue. (D and E) Densitometric analysis of protein bands in liver tissue. (F) The level of IL-22 in serum was detected by ELISA. (G) Western blot analysis was performed to quantify the levels of α7nAChR and IL-22RA1 in L-02 cells. (H and I) Densitometric analysis of protein bands in L-02 cells. (J) The level of IL-22 in cell supernatant was detected by ELISA. (K and L) qRT-PCR was used to measure the levels of α7nAChR and IL-22 in L-02 cells. (ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001).
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Fig. 2. The effect of PNU282987 <t>on</t> <t>IL-22</t> production. (A) The expression level of α7nAChR in liver tissue was detected by immunohistochemistry (scale, 100 μm, 50um). (B) The integrated optical density (IOD) of α7nAChR. (C) Western blot analysis was performed to quantify the levels of α7nAChR and IL-22RA1 in liver tissue. (D and E) Densitometric analysis of protein bands in liver tissue. (F) The level of IL-22 in serum was detected by ELISA. (G) Western blot analysis was performed to quantify the levels of α7nAChR and IL-22RA1 in L-02 cells. (H and I) Densitometric analysis of protein bands in L-02 cells. (J) The level of IL-22 in cell supernatant was detected by ELISA. (K and L) qRT-PCR was used to measure the levels of α7nAChR and IL-22 in L-02 cells. (ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001).
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Fig. 2. The effect of PNU282987 <t>on</t> <t>IL-22</t> production. (A) The expression level of α7nAChR in liver tissue was detected by immunohistochemistry (scale, 100 μm, 50um). (B) The integrated optical density (IOD) of α7nAChR. (C) Western blot analysis was performed to quantify the levels of α7nAChR and IL-22RA1 in liver tissue. (D and E) Densitometric analysis of protein bands in liver tissue. (F) The level of IL-22 in serum was detected by ELISA. (G) Western blot analysis was performed to quantify the levels of α7nAChR and IL-22RA1 in L-02 cells. (H and I) Densitometric analysis of protein bands in L-02 cells. (J) The level of IL-22 in cell supernatant was detected by ELISA. (K and L) qRT-PCR was used to measure the levels of α7nAChR and IL-22 in L-02 cells. (ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001).
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Fig. 2. The effect of PNU282987 on IL-22 production. (A) The expression level of α7nAChR in liver tissue was detected by immunohistochemistry (scale, 100 μm, 50um). (B) The integrated optical density (IOD) of α7nAChR. (C) Western blot analysis was performed to quantify the levels of α7nAChR and IL-22RA1 in liver tissue. (D and E) Densitometric analysis of protein bands in liver tissue. (F) The level of IL-22 in serum was detected by ELISA. (G) Western blot analysis was performed to quantify the levels of α7nAChR and IL-22RA1 in L-02 cells. (H and I) Densitometric analysis of protein bands in L-02 cells. (J) The level of IL-22 in cell supernatant was detected by ELISA. (K and L) qRT-PCR was used to measure the levels of α7nAChR and IL-22 in L-02 cells. (ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001).

Journal: Cytokine

Article Title: The protective effect of the vagus nerve-α7nAChR-IL-22 pathway on acute liver injury.

doi: 10.1016/j.cyto.2024.156840

Figure Lengend Snippet: Fig. 2. The effect of PNU282987 on IL-22 production. (A) The expression level of α7nAChR in liver tissue was detected by immunohistochemistry (scale, 100 μm, 50um). (B) The integrated optical density (IOD) of α7nAChR. (C) Western blot analysis was performed to quantify the levels of α7nAChR and IL-22RA1 in liver tissue. (D and E) Densitometric analysis of protein bands in liver tissue. (F) The level of IL-22 in serum was detected by ELISA. (G) Western blot analysis was performed to quantify the levels of α7nAChR and IL-22RA1 in L-02 cells. (H and I) Densitometric analysis of protein bands in L-02 cells. (J) The level of IL-22 in cell supernatant was detected by ELISA. (K and L) qRT-PCR was used to measure the levels of α7nAChR and IL-22 in L-02 cells. (ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001).

Article Snippet: Recombinant human IL-22 protein (Cat No. HY-P7039, MCE).

Techniques: Expressing, Immunohistochemistry, Western Blot, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR

Fig. 4. The effect of PNU282987 on liver apoptosis. (A) Hepatocyte apoptosis was determined by TUNEL staining.(B) Results of TUNEL staining analysis. (C) Western blot analysis was performed to quantify the levels of Bcl-2 and Bax in liver tissue. (D and E) Grayscale analysis of protein bands in liver tissue was conducted. (F) Western blot analysis was performed to quantify the levels of Bcl-2 and Bax in L-02 cells. (G and H) Grayscale analysis of protein bands in L-02 cells was conducted. (I) The level of IL-22 in cell supernatant was detected by ELISA. (ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).

Journal: Cytokine

Article Title: The protective effect of the vagus nerve-α7nAChR-IL-22 pathway on acute liver injury.

doi: 10.1016/j.cyto.2024.156840

Figure Lengend Snippet: Fig. 4. The effect of PNU282987 on liver apoptosis. (A) Hepatocyte apoptosis was determined by TUNEL staining.(B) Results of TUNEL staining analysis. (C) Western blot analysis was performed to quantify the levels of Bcl-2 and Bax in liver tissue. (D and E) Grayscale analysis of protein bands in liver tissue was conducted. (F) Western blot analysis was performed to quantify the levels of Bcl-2 and Bax in L-02 cells. (G and H) Grayscale analysis of protein bands in L-02 cells was conducted. (I) The level of IL-22 in cell supernatant was detected by ELISA. (ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).

Article Snippet: Recombinant human IL-22 protein (Cat No. HY-P7039, MCE).

Techniques: TUNEL Assay, Staining, Western Blot, Enzyme-linked Immunosorbent Assay

Fig. 5. The effects of exogenous IL-22 and IL-22BP on the regeneration and apoptosis of L-02 cells. (A) In the IL-22 group, the levels of PCNA, Bcl-2 and Bax in L-02 cells were assessed using Western blotting. (B,C and D) The grayscale analysis of protein bands in L-02 cells. (E) In the IL-22BP group, the levels of PCNA, Bcl-2 and Bax in L-02 cells were assessed using Western blotting. (F,G and H) The grayscale analysis of protein bands in L-02 cells. (*P < 0.05, **P < 0.01).

Journal: Cytokine

Article Title: The protective effect of the vagus nerve-α7nAChR-IL-22 pathway on acute liver injury.

doi: 10.1016/j.cyto.2024.156840

Figure Lengend Snippet: Fig. 5. The effects of exogenous IL-22 and IL-22BP on the regeneration and apoptosis of L-02 cells. (A) In the IL-22 group, the levels of PCNA, Bcl-2 and Bax in L-02 cells were assessed using Western blotting. (B,C and D) The grayscale analysis of protein bands in L-02 cells. (E) In the IL-22BP group, the levels of PCNA, Bcl-2 and Bax in L-02 cells were assessed using Western blotting. (F,G and H) The grayscale analysis of protein bands in L-02 cells. (*P < 0.05, **P < 0.01).

Article Snippet: Recombinant human IL-22 protein (Cat No. HY-P7039, MCE).

Techniques: Western Blot